In Vitro

SPF Determination

Florida Skincare Testing performs a variety of tests associated with sunscreen formulas. The IMS division utilizes our proprietary Vitro-Skin® substrates. This pre-clinical method allows a rapid understanding of the SPF value before a full clinical test is conducted. Other in vitro tests with synthetic substrates can also accelerate your development process.

  • Provide exploratory SPF estimates to guide formulation decisions.
  • Reduce the need for multiple in vivo iterations.
  • Support comparative testing between prototypes.


While not a regulatory substitute for in vivo SPF testing (ISO 24444, FDA), in vitro methods using Vitro-Skin are invaluable for early screening and formulation optimization.

The benefit of using Vitro-Skin over the traditional PMMA plates is improved accuracy in the results when screening in-vitro. While using Vitro-Skin the scientist has the ability to apply the correct and standardized dose of 2mg/cm2 as required in the in-vivo studies, whereas the dose needs to be reduced much lower when using PMMA. PMMA which is a type of plastic does not absorb the applied product, unlike Vitro-Skin which mimics the skin composition and has similar absorption properties of human skin.